Hydrogen peroxide generation by higher plant mitochondria oxidizing complex I or complex II substrates
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چکیده
منابع مشابه
Pyruvate dehydrogenase complex from higher plant mitochondria and proplastids: kinetics.
A steady-state kinetic analysis has been performed on the pyruvate dehydrogenase complex from pea (Pisum sativum L.) mitochondria and castor bean (Ricinus communis L.) proplastids. Substrate interaction kinetics for all substrates gave parallel lines consistent with a multisite ping-pong mechanism. Product inhibition studies showed uncompetitive inhibition between acetyl-CoA and pyruvate and co...
متن کاملPyruvate dehydrogenase complex from higher plant mitochondria and proplastids.
The pyruvate dehydrogenase complex from pea (Pisum sativum L.) mitochondria was purified 23-fold by high speed centrifugation and glycerol gradient fractionation. The complex had a s(20,w) of 47.5S but this is a minimal value since the complex is unstable. The complex is specific for NAD(+) and pyruvate; NADP(+) and other keto acids give no reaction. Mg(2+), thiamine pyrophosphate, and cysteine...
متن کاملPyruvate dehydrogenase complex from higher plant mitochondria and proplastids: regulation.
The activity of the pyruvate dehydrogenase complex from pea (Pisum sativum L.) mitochondria is inhibited when MgATP is added to the reaction mixture; 50% inhibition occurs at 4 mm ATP. The inhibition does not increase with time and is higher in the more highly purified preparations. Crude preparations of the complex show a time-dependent inactivation when incubated with 7.5 mm MgATP alone but t...
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De®ciency of complex I in the respiratory chain and oxidative stress induced by hydrogen peroxide occur simultaneously in dopaminergic neurones in Parkinson's disease. Here we demonstrate that the membrane potential of in situ mitochondria (DCm), as measured by the ̄uorescence change of JC-l (5,5 0,6,6 0-tetrachloro-1,1,3,3 0-tetraethylbenzimidazolylcarbocyanine iodide), collapses when isolated...
متن کاملGeneration of Hydrogen Peroxide by Plant Peroxidases Mediated Thiol Oxidation
OBINGER C , BURNER U. & EBERMANN R. 1997. Generation of hydrogen peroxide by plant peroxidases mediated thiol oxidation. Phyton (Horn, Austria) 37 (3): (219) (226). Thiol oxidation by plant peroxidases is an oxygen consuming process independent of hydrogen peroxide addition. The in vitro system consists of two interdependent reactions, i.e. hydrogen peroxide mediated oxidation of thiols via the...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1999
ISSN: 0014-5793
DOI: 10.1016/s0014-5793(99)00616-x